Preparation of Caprine and Swine Placental Peptide and Studies on Its Biological Activity

The distilled caprine and swine placental peptides were prepared by ultrafilter and the molecular weight of caprine placental peptide which was 4,386Da was mensurated by improved Tricine-SDS-PAGE eletrophoresis with urea.Their absorbance peaks which were respectively 225.4nm and 209.6nm were got by ultraviolet scanning.Their immnoregulatory effects were mensurated by MTT Colorimetric Assay after establishing a model of in vitro immunosuppressive lymphocytes of rabbit.The results in the experiment showed that the bovine and caprine placental peptides could improve immunosuppressive T lymphocyte transformation rate.The immunosuppressive lymphocyte transformation rates in groups with the caprine placental peptides of 1:10~2,1:10~3and 1:10~4(P0.01)and with the swine placental peptides of 1:10~2,1:10~3(P0.01)increased more remarkably than that in immunosuppression group.