Full-length cloning and bioinformatics analysis of TaCIPK31 gene in wheat

Based on the wheat CIPK EST sequence of TC95390,we got the 3′-end and 5′-end using RACE(rapid-amplification of cDNA ends) method from wheat cultivar Shi 4185 which has higher drought-resistance and salt-resistance.The whole sequence of gene was analyzed and named as TaCIPK31 by sequence homology and deposited in GenBank database of accession number JX625142.1.Bioinformatics analysis was undergone meanwhile.The results showed that the ORF length of TaCIPK31 was 1 350 bp and encoded 449 amino acids.The pI and Mw of TaCIPK31 were 8.27 and 50.9 kD,respectively.TaCIPK31 was a hydrophilic stable protein while there was only one significant hydrophobic peak and one transmembrane peptide.The TaCIPK31 protein contained a catalytic Ser/Thr protein domain in its N-terminal region and a regulatory domain with NAF motif at its C-terminus,which is in line with the typical CIPK structural characteristics.The amino acids sequence analysis showed that TaCIPK31 has higher homology with HvCIPK31,OsCIPK3 and ZmCIPK31.