Electronic Clone and Bioinformatics Analysis of DREB Gene from Wheat

The full-length cDNA sequence of DREBgene was obtained by in silico cloning using NP_195489sequence from Arabidopsis as the probe sequence.Some characters of the clone gene encoding amino acid,including the composition of amino acid sequence,physical and chemical properties,secondary and tertiary structure of protein plus functional domains,were analyzed by bioinformatics tools.The results showed that the full-length gene from wheat was 2008 bp,including one 639 bp open reading frame(ORF)which encodes a polypeptide of 212 amino acids.The encoded protein of the clone gene,with several conserved AP2 domain sequences,was soluble and located in nucleus.Electronic expression analysis revealed that the clone gene was constitutively expressed in the wheat young ear,seedling,semet,root,leaf and corol,and the expression of this gene in the root and leaf of wheat was much higher than that in all the other types of wheat tissues.