Research of expression and clinical significance of p53,PCNA and PTEN in gastric cancer

Objective To detect the expression and clinical significance of p53,PCNA(proliferating cell nuclear antigen) and PTEN(phosphatase and tensin homolog deleted on chromosome ten) protein in gastric cancer tissues.Methods To detect expression of p53,PCNA and PTEN in gastric cancer tissues by immunohistochemical staining methods in the follow-up of gastric cancer patients after surgical treatment.Results Positive rate of p53 expression was 58.2%;Strong positive rate of PCNA expression was 52.7%;Negative rate of PTEN expression was 52.7%.The p53,PCNA and PTEN expression were related with the depth of tumor invasion and lymph node metastasis(all P 0.05) and were not related with the degree of tumor differentiation(all P 0.05).Gastric cancer patients with p53 expression had less probability of 5-year survival than patients with negative expression;gastric cancer patients with strong PCNA expression had less probability of 5-year survival than patients with weakly expression;gastric cancer patients with PTEN expression had more probability of 5 years survival than patients with negative expression.The differences were all statistically significant(P 0.05).Conclusions Expression of p53,PCNA and PTEN are all related to the depth of tumor invasion and metastasis of lymph node.Gastric cancer patients with positive expression of p53,strong positive of expression PCNA or negative expression of PTEN have poor prognosis.The detections of p53,PCNA and PTEN expression in gastric cancer specimens might be used as one of the indicators to determine the prognosis of gastric cancer patients.