Research on the Technology of the Detoxification of Rapeseed Meal and the Isolation of Its Protein

[Objective] The theoretic guidance for the development and utilization of rapeseed meal was provided through the research on the extracting technology of protein from rapeseed meal.[Method] The double-low rapeseed meal detoxified being taken as raw material,the effect of the different factors on the extracting rate of protein was experimented and the affecting factor was optimized with response surface method.[Result] The single-factor experimental result indicated that the best extraction technology of the protein from rapeseed meal was the pH value was 13;the temperature,40 ℃;the ratio of material to liquid(g/ml),1:12 and the extraction time,40 minutes for 3 times' extraction.Based on the result,the level of the pH value,the extraction temperature and the ratio of material to liquid was further optimized with response surface method and while the argument was the pH value of 12.9,the temperature of 40.8 ℃ and the ratio of material to liquid of 1:12.6,which was produced by means of the quadratic regression equation,the optimum prediction yield of the protein from rapeseed meal was 57.73%.[Conclusion] The rapeseed meal could certainly be detoxified in the treatment of acetone-ethanol solution.The guidance of the mathematical model,formed through response surface method,for the prediction of all factors affecting the extracting efficiency of the protein from rapeseed meal was feasible.