Optimization of Technology for Extracting Alkali-soluble Polysaccharides from Rapeseed Meal and Study on Anti-oxidative Effect in vitro
WU Mou-cheng
Abstract
WU Mou-cheng
Abstract
In this paper, sodium hydroxide was used as extracting agent from rapeseed meal. Three levels of the factors including extraction temperature, concentration of NaOH and extraction time were selected for the central composite design experiment. The quadric regression model for the yield of polysaccharides was established. Then the optimum combination was obtained by SAS analysis and response surface analysis. Experimental results indicated that when the extraction parameters are composed of 93.7 ℃, 1.125 mol/L and 2.3 h, the yield of polysaccharides is maximum to 11.59%. At last the study of polysaccharide from rapeseed meal anti-oxidation was conducted, and the medical valve of rapeseed meal was proved.
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In this paper, sodium hydroxide was used as extracting agent from rapeseed meal. Three levels of the factors including extraction temperature, concentration of NaOH and extraction time were selected for the central composite design experiment. The quadric regression model for the yield of polysaccharides was established. Then the optimum combination was obtained by SAS analysis and response surface analysis. Experimental results indicated that when the extraction parameters are composed of 93.7 ℃, 1.125 mol/L and 2.3 h, the yield of polysaccharides is maximum to 11.59%. At last the study of polysaccharide from rapeseed meal anti-oxidation was conducted, and the medical valve of rapeseed meal was proved.
Key concepts: Rapeseed, Polysaccharide, Response surface methodology, Meal, Extraction (chemistry), Sodium hydroxide, Food science, Chemistry