Optimization of Protein Extraction Process and Scavenging Function of DPPH Radical From Rapeseed Meal

Taking hot-pressed rapeseed meal as material,the optimum protein extraction process by alkali extraction and acid precipitation was studied.The results showed that the optimal conditions of extraction were follows:pH 11.5,temperature 55℃,ratio of material to liquid 1∶15g/mL,ultrasonic power 80W,extraction time 100min,3times of extraction.Under these conditions,the extraction yield of soluble protein from rapeseed meal was 28.21%,the rapeseed protein yield of end product was 23.70%.The albumin and globulins by isolation of rapeseed protein had powerful scavenging efficiency to DPPH radical,the clearance rate were 31.09% and 29.08% respectively.