Accommodation of Xiongzhen Ointment on CRP,TNF-ɑ and the Effects on Skin Wound Healing of Rats

Objective:To observe the effect of Xiongzhen Ointment in micro-vessels and number of fibroblasts in rat cutaneous incised wound healing and investigate the mechanism of accelerating the rat back wound healing.Methods:After setting up the model,the rats were randomly assigned to 4 groups:model group,bFGF-ESSEX group(western medicine control group),Xiongzhen Ointment group,bFGF-ESSEX + Xiongzhen Ointment group.The medicine were attach to the skin twice a day.After setting up models for 3,7and 14 days,the rats were killed to perform immune-histochemistry study.S-P method of immune-histochemistry was used to detect CD34,number of fibroblasts of the local granulation tissue.We used image analysis equipment and computer software to analyze the image of each group.The data were expressed as Mean±SD and was analyzed by SPSS 18.0.Results:The number intensity of granulation tissue were significantly better in Xiongzhen Ointment group than model group in all three time points(P0.01).The improvement of number of fibroblasts was significantly better in Xiongzhen ointment group than model group in all three time points.Conclusion:The Xiongzhen Ointment can accelerate the healing of skin trauma of the rats and improve the quality of healing.This may be related to microvessels improvement and fibroblasts numbers of granulation tissue.