Effects of Fuhuang Shengji Yuchuang Ointment on Expression of Smad3 mRNA and Smad7 mRNA in Granulation Tissue of Wound in Rats with Diabetes

Objective To observe the effects of Fuhuang Shengji Yuchuang(FHSJYC) ointment,a compound traditional Chinese herbal medicine,on the expression of samd3 and samd7 in granulation tissue in wistar rats with diabetes.Methods The male wistar rats were randomly divided into three groups including wound control group,diabetes models group and FHSJYC ointment group.Diabetes models were induced by injection of 1.5% alloxan and oral gavage of 5% glucose,and skin wound was made in rats of the normal saline(NS) group and the FHSJYC ointment group.Skin wounds of the rats in the FHSJYC ointment group were treated with FHSJYC ointment gauze dressing,while those in the NS group were treated with NS gauze dressing once daily.The rats were executed in turn on the third day and the eleventh day of the treatment,and the changes of smad3,smad7 in the wound granulation tissue were observed by fluorescent real time quantitative polymerase chain reaction.Results Compared with the NS group,the wound closure index in the FHSJYC ointment group was increased(P0.05).After three-day treatment,the expression of smad3 mRNA in the FHSJYC ointment group was higher than those in the NS group(P0.05),similar with those in the wound control group,while the expression of smad7 mRNA showed no significant differences among the three groups.After 11-day treatment,the expression of smad3 mRNAs in the FHSJYC ointment group was lower than those in the wound control group(P0.05),similar with those in the NS group.The expression of smad7 mRNA in the FHSJYC ointment group was higher than that in the NS group(P0.05),similar with that in the wound control group.Conclusion FHSJYC ointment can affect the process of wound healing by promoting and regulating the expression of smad3 and smad7 mRNAs.These results show effects in accelerating wound healing and minimizing scar proliferation in uncurable wound.