Chemical Constituents of Vitex negundo var. cannabifolia Fruits

Objective: The purpose of this article was to study the chemical constituents of Vitex negundo var. cannabifolia fruits. Method: Constituents were separated and repeatedly purified through column chromatography on silica gel column,ODS and preparative HPLC. Their structures were elucidated by spectral analysis. Result: Eight compounds were obtained from the ethyl acetate extracts,their structures were identified as: casticin( 1), detetrahydroconidendrin( 2), 6-hydroxy-4β-( 4-hydroxy-3-methoxyphenyl)-3α-hydroxymethyl-7-methoxy-3,4-dihydro-2-naphthaldehyde( 3),ethyl p-hydroxybenzoate( 4),5,4'-dihydroxy-3,6,7,8,3'-pentamethoxyflavone( 5),5-hydroxy-6,7,3',4'-tetramethoxyflavone( 6),5,4'-dihydroxy-6,7,8,3'-tetramethoxyflavone( 7),isolariciresinol( 8). Conclusion: Compounds 4,5 and 7 were obtained from this genus for the first time. Compound 2 was obtained from this plant for the first time.