Molecular typing by PFGE and serotyping of vibrio parahaemolyticus from food poisoning

Objective To Study serotypes,virulence gene and molecular typing of Vibrio parahaemolyticus isolated from one food poisoning.Methods Six strains of V.parahaemolyticus were identified and typed using specific antisera of O and K.Multiplex PCR method were operated for testing presence of tdh and trh.The genomic DNA of isolates was digested with Sfi I,then run PFGE.The DNA fingerprints were analyzed by Bionumerics software to perform clustering.Results Serotypes of 6 isolates were O1:K36 and there were gene tdh without trh in 6 isolatess.The DNA fingerprints of 5 isolates are identical with a similar rate of 98%.Conclusion The cases of food poisoning are caused by the same clone group of V.parahaemolyticus.PFGE is a good method for molecular typing of V.parahaemolyticus and could be effectively applied to trace the suspected source in food-poisoning.