2001Sichuan Nongye Daxue xuebaoRequires access

Primary Study on Callus Culture in Mature Embryo of New Wheat Varieties R_(59)

Tang Zong

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Abstract

Mature embryos of new wheat variety R 59 were used as explant for culture in vitro in three kinds of culture medium. The result shows that the ratio of callus induced is greatly different and the ratio of callus induced from the better culture medium (Ms+2,4-D2 0mg/L+KT0 5mg/L) is 92%~93 3%. The result also shows that embryonic bud is disadvantageous to inducing embryonic callus.

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What this paper is about

Mature embryos of new wheat variety R 59 were used as explant for culture in vitro in three kinds of culture medium. The result shows that the ratio of callus induced is greatly different and the ratio of callus induced from the better culture medium (Ms+2,4-D2 0mg/L+KT0 5mg/L) is 92%~93 3%. The result also shows that embryonic bud is disadvantageous to inducing embryonic callus.

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Available abstract

Mature embryos of new wheat variety R 59 were used as explant for culture in vitro in three kinds of culture medium. The result shows that the ratio of callus induced is greatly different and the ratio of callus induced from the better culture medium (Ms+2,4-D2 0mg/L+KT0 5mg/L) is 92%~93 3%. The result also shows that embryonic bud is disadvantageous to inducing embryonic callus.

Key concepts: Callus, Explant culture, Embryo, Biology, Embryonic stem cell, Botany, Tissue culture, Embryo culture

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