Study on in vitro culture of mature embryo from different wheat varieties

Taking Hubei province current released species E-en NO.4,E-mai 14,and Y u-mai 70 wheat mature embryo as explants, adoping basic culture media MS,making up twenty culture media combined by varied hormone contents and salt stress, aut hor studies the impact of varied genotypes,2,4-D consistency and NaCl content on mature embryo's callus formation. The results show that callus inducing ratioe s are quite different with different genotypes,2,4-D contents exist variation wi th the callus formation,E-en NO.4 has the highest callus inducing ratio,on MS+2, 4-D 8.0mg·L-1 culture media. Callus inducing ratio reaches 100%. Callus inducin g ratio E-mail 70 reaches 90% on the MS+2,4-D 6.0mg·L-1 culture media, E-mail 1 4 is weaker and it's callus inducing ratio reaches 83.3% MS+2,4-D on 8.0mg·L-1 culture media.In the extent of 4.0～8.0mg·L-1,2,-4-D content has positive dist inctively restrain callus formation and the E-en NO.4 has a strong salt toleranc e.