Study on Optimization of the Extraction Technology of Lycium Barbarum Polysaccharide by Response Surface Methodology

In this study,the method of polysaccharide extraction was hot water extraction and discussing the crushing of raw materials,decolor,pre-soaked in the three factors of pre-experimental extraction to the effect of the follow-up.With anthrone-sulfuric acid method,the content of LBP was tested under the 620nm and the yield was calculated.Box-Behnken statistical design technology was used to optimize the method of LBP extraction.With the response surface optimization,the optimum conditions were: extraction temperature 87℃,liquid ratio 1:26,extraction time 7h.On the condition,the highest yield of LBP was 8.2%.The results were not only had the best extraction conditions for LBP,but also the results of response surface design experiment was much higher the traditional orthogonal in the degree of accuracy.The reference was provided for flarge-scale factory production in the future.