Dissolution of baicalin from Sanhuang Tablets

Objective To review the dissolution behavior of baicalin from Sanhuang Tablets of different batches produced by different producers in market.Method The content of baicalin was determined by using HPLC.The procedure of HPLC was performed on the chromatographic column of Dimansil C18(250 mm×4.6 mm,5μm).The mobile phase was CH3OH-0.1% H3PO4(51∶49),the detection wavelength was 280 nm and flow velocity was 1 mL/min.The determination conditions of dissolution were selected,accumulation dissolution curves were calculated,drawn and compared by similarity factor values(f2).Result The determination conditions of dissolution included paddle method with the rotating speed of 100 r/min and phosphate buffer(pH=7.2) as dissolution medium.The result of determination showed that the similarity factor values(f2) of baicalin dissolution curves of different batches of Sanhuang Tablets were all less than 50.Conclusion There was significant difference in baicalin dissolution among Sanhuang Tablets of different batches produced by different producers.