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2004•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Baicalin in Kangbingdu Capsules by HPLC

Ling Zhang

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Abstract

Objective:To establish a determination method for baicalin in Kangbingdu Capsules. Methods: HPLC with YWG C (18)(250×4.6, 10 μm) column was used. The mobile phase was methanol-water H 3PO 4(47∶53∶0.2). The detection wavelength was at 280 nm. Results: There was good linear relationship between 0.099 5～0.796 0 μg, r=(0.999 98). The average recovery was 97.40% and RSD was 0.81%. Conclusion: This method is simple, quick, accurate and with good reproducibility and can be used for the quality control of Kangbingdu Capsules.

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What this paper is about

Objective:To establish a determination method for baicalin in Kangbingdu Capsules. Methods: HPLC with YWG C (18)(250×4.6, 10 μm) column was used. The mobile phase was methanol-water H 3PO 4(47∶53∶0.2). The detection wavelength was at 280 nm. Results: There was good linear relationship between 0.099 5～0.796 0 μg, r=(0.999 98). The average recovery was 97.40% and RSD was 0.81%. Conclusion: This method is simple, quick, accurate and with good reproducibility and can be used for the quality control of Kangbingdu Capsules.

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Available abstract

Objective:To establish a determination method for baicalin in Kangbingdu Capsules. Methods: HPLC with YWG C (18)(250×4.6, 10 μm) column was used. The mobile phase was methanol-water H 3PO 4(47∶53∶0.2). The detection wavelength was at 280 nm. Results: There was good linear relationship between 0.099 5～0.796 0 μg, r=(0.999 98). The average recovery was 97.40% and RSD was 0.81%. Conclusion: This method is simple, quick, accurate and with good reproducibility and can be used for the quality control of Kangbingdu Capsules.

Key concepts: Baicalin, Chromatography, Reproducibility, High-performance liquid chromatography, Chemistry, Linear relationship, Mathematics, Statistics

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