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2010•Research and Practice on Chinese MedicinesRequires access

Determination of the Baicalin Content in Biyanling Capsules by HPLC

Cai Hai-min

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Abstract

Objective To establish HPLC method for the content determination of baicalin in Biyanling capsules.Methods RP- HPLD was used on Symmetry C18 column(3.9 mm×150 mm,5 μm)with methanol- watwr-glacial acetic acid(47:52:1) as mobile phase and the flow rate of 1.0 mL·min-1.The detection wavelength was at 280 nm .The external standard method was employed for quantitative analysis.Results The linear range of baicalin was in the range of 0.346~1.73 μg( r = 0.999 7) with average recovery rate of 98.96 % (RSD=2.40 %,n=5).Conclusion The method is simple,accurate and reproducible,and it can be used for the quality control of baicalin in Biyanling capsules.

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What this paper is about

Objective To establish HPLC method for the content determination of baicalin in Biyanling capsules.Methods RP- HPLD was used on Symmetry C18 column(3.9 mm×150 mm,5 μm)with methanol- watwr-glacial acetic acid(47:52:1) as mobile phase and the flow rate of 1.0 mL·min-1.The detection wavelength was at 280 nm .The external standard method was employed for quantitative analysis.Results The linear range of baicalin was in the range of 0.346~1.73 μg( r = 0.999 7) with average recovery rate of 98.96 % (RSD=2.40 %,n=5).Conclusion The method is simple,accurate and reproducible,and it can be used for the quality control of baicalin in Biyanling capsules.

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Available abstract

Objective To establish HPLC method for the content determination of baicalin in Biyanling capsules.Methods RP- HPLD was used on Symmetry C18 column(3.9 mm×150 mm,5 μm)with methanol- watwr-glacial acetic acid(47:52:1) as mobile phase and the flow rate of 1.0 mL·min-1.The detection wavelength was at 280 nm .The external standard method was employed for quantitative analysis.Results The linear range of baicalin was in the range of 0.346~1.73 μg( r = 0.999 7) with average recovery rate of 98.96 % (RSD=2.40 %,n=5).Conclusion The method is simple,accurate and reproducible,and it can be used for the quality control of baicalin in Biyanling capsules.

Key concepts: Baicalin, Chromatography, High-performance liquid chromatography, Acetic acid, Chemistry, Content determination, Linear range, Volumetric flow rate

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