Enzymatic Hydrolysis of Rice Straw Pretreated by Irradiation at High Solid Loading
Chen Lian
Abstract
Chen Lian
Abstract
The effect of high dose irradiation on polysaccharides of rice straw was studied. The feasibility of the achievement of high-concentration sugar by the way of improving substrate concentration through fed-batch enzymatic hydrolysis process of irradiated rice straw,was then investigated. The result showed that the polysaccharides content of irradiated rice straw decreased and the water-soluble sugar increased from 0. 7% to 13. 4%. By the way of fed-batch enzymatic hydrolysis,the solid loading could reach 40%( mass fraction) in the enzymatic hydrolysis system. And 104. 6 g / L glucose concentration as well as 77. 1%cellulose conversion were obtained by this process under the condition of 32. 8 FUP / g( calculated by cellulose) cellulase loading.
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The effect of high dose irradiation on polysaccharides of rice straw was studied. The feasibility of the achievement of high-concentration sugar by the way of improving substrate concentration through fed-batch enzymatic hydrolysis process of irradiated rice straw,was then investigated. The result showed that the polysaccharides content of irradiated rice straw decreased and the water-soluble sugar increased from 0. 7% to 13. 4%. By the way of fed-batch enzymatic hydrolysis,the solid loading could reach 40%( mass fraction) in the enzymatic hydrolysis system. And 104. 6 g / L glucose concentration as well as 77. 1%cellulose conversion were obtained by this process under the condition of 32. 8 FUP / g( calculated by cellulose) cellulase loading.
Key concepts: Chemistry, Cellulase, Cellulose, Hydrolysis, Enzymatic hydrolysis, Straw, Sugar, Polysaccharide