Simultaneous Determination of Four Effective Components in Rose Oral Liquid by High Performance Liquid Chromatography

An analytical method for the simultaneous determination of four effective components(Gallic acid,Rutin,Quercetin,Kaempferol)in Rose Oral Liquid was established using high performance liquid chromatography(HPLC).The separation was performed on an Agilent C18 column(250×4.6mm,5μm)at 30℃and 0.5% phosphoric acid-methanol as mobile phases with gradient elution at a flow rate of 0.8mL/min,and the detection wavelength was 260nm.Good linearity was obtained between the peak area and injected amount when the amount was 40.7-244.2μg·mL-1 for Gallic acid,25.0-150.0μg·mL-1for Rutin,13.3-80μg·mL-1 for Quercetin and 2.69-16.2μg·mL-1 for Kaempferol.The correlation coefficient of each component was 0.9997,0.9993,0.9998 and 0.9994,respectively.Average recoveries of the four components were 99.2%,101.2%,98.8%,99.2%.RSD of each group were 0.60%,1.02%,0.62%,0.57%(n=3).Four effective components in Rose Oral Liquid were simultaneously determined,and the method was convenient and accurate.