Determination of Quercetin and Kaempferol in Mulberry Oral Liquid by HPLC

Objective: To develop a high performance liquid chromatographic method for the determination of quercetin and kaempferol in mulberry oral liquid Method: The optimized method was achieved for the separation and detection of quercetin and kaempferol us ing Symmetry C18 column(150mm×4.6mm 5μm) as the stationary phase,methanol-0.4% aqueous phosphoric acid solution(60 40) as the mobile phase at a flow rate of 0.5 mL min-1,370 nm as the detection wavelength.The main,which influenced the Sample hydro lysis procedure,were intensively explored.Result: The optimized hydrolysis procedure was that the sample was hydrolyzed 2.0 h with 2.0 mol L-1 hydrochloric acid methanol at 90 ℃.Quercetin and kaempferol showed good relationship at the range of 0.18-35.28 mg L-1,0.19-38.22mg L-1,respectively.Both of the correlation coefficients of the calibration curves were 0.999 8.The average recoveries of quercetin and kaempferol were 99.43% and 99.19% with relative standard derivation(RSD) of 1.16% and 2.04%.Conclusion: The re sults showed that the method is simple,accurate and repeatable and it is suitable for quality control of mulberry oral liquid.