Experimental Treatment of Diabetic Mice with Microencapsulated Rat Islet Cells Transplantation

To observe treatment effects of diabetic mice with microcapsulated and non-microcapsulated rat islet cell transplantation,pancreas of SD rat was perfused with collagenase through cloledchus,and then the pancreatic tissues were isolated and digested.Histopaque-1077 was used to purify the digested pancreas.Islet cells were collected and implanted into the peritoneal cavity of diabetic mice.The isolated islets had a response upon glucose stimulation.When the microcapsulated islets and non-microcapsulated islets were transplanted into diabetic mices the high blood glucose level could be decreased to normal.The normal blood glucose level in the diabetic mice transpanted with microcapsulated islets could be maintained for over 30 days,but it could be mainlained only for 2-3 days in the diabetic mice transplanted with non-microcapsulated islets.Thus it is believed that microcapsulated islet cell transplantation exerts good effect on diabetic mice and the microcapsules possessed good immunoisolating function.