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Determination of Emodin and Chrysophanol in Cuochuang Ointment by HPLC

Guan Ren-jie

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Abstract

Objective:To determine the contents of emodin and chrysophanol in Cuochuang Ointment by HPLC.Methods:C_(18) column was used.The mobile phase consisted of methanol-0.1% phosphoric acid solution(85∶15).The detected wavelength was set at 440 nm.Results:The linearity of this method was good.In the range of emodin was(0.024～0.3)μg(r=1);chrysophanol was(0.02～0.25)μg(r=1).The recoveries of emodin and chrysophanol were 99.5% and 97.4%,RSD were 2.98% and 2.33%,respectively.Conclusion:The method is accurate,precise and is suitable to detect emodin and chrysophanol in Cuochuang Ointment.

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What this paper is about

Objective:To determine the contents of emodin and chrysophanol in Cuochuang Ointment by HPLC.Methods:C_(18) column was used.The mobile phase consisted of methanol-0.1% phosphoric acid solution(85∶15).The detected wavelength was set at 440 nm.Results:The linearity of this method was good.In the range of emodin was(0.024～0.3)μg(r=1);chrysophanol was(0.02～0.25)μg(r=1).The recoveries of emodin and chrysophanol were 99.5% and 97.4%,RSD were 2.98% and 2.33%,respectively.Conclusion:The method is accurate,precise and is suitable to detect emodin and chrysophanol in Cuochuang Ointment.

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Available abstract

Objective:To determine the contents of emodin and chrysophanol in Cuochuang Ointment by HPLC.Methods:C_(18) column was used.The mobile phase consisted of methanol-0.1% phosphoric acid solution(85∶15).The detected wavelength was set at 440 nm.Results:The linearity of this method was good.In the range of emodin was(0.024～0.3)μg(r=1);chrysophanol was(0.02～0.25)μg(r=1).The recoveries of emodin and chrysophanol were 99.5% and 97.4%,RSD were 2.98% and 2.33%,respectively.Conclusion:The method is accurate,precise and is suitable to detect emodin and chrysophanol in Cuochuang Ointment.

Key concepts: Emodin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Methanol, Organic chemistry

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