The improvements in establishment of rat orthotopic pulmonary allograft transplantation model

Objective With the improved cuff-like vessel anastomosis technique to establish the rat orthotopic left pulmonary allograft transplantation model.Methods Forty rats were randomly divided into donor and recipient groups.The anastomosis of pulmonary artery,vein and bronchus was performed by using the improved cuff-like vessel anastomosis technique.Results In 20 cases of operations,time of dornor lung perfusion-picking,donor lung vessel anastomosis and recipient vessel anastomosis was (8±1),(6±2),(11±2) min respectively.The total ischemic time was (36±7) min,and the mean operative time was (59±4) min.The operation successful rate was 90 % (18/20),and survivial rate was 100 %.Conclusion The improved cuff-like vessel anastomosis technique for establishment of rat orthotopic pulmonary allograft transplantation model is simple and applicable.