To establish rat orthotopic left pulmonary allograft transplantation by improving the "cuff-like" vessel anastomosis technique

OBJECTIVE To establish a more stable and effective rat orthotopic left pulmonary allograft transplantation models.METHODS 40 rats were randomly divided into two groups:donors group and recipients group.Pulmonary arteries and veins were anastomosed by traditional cuff technique.Bronchi was reconstructed by stent technique.RESULTS 20 cases of rat orthotopic left pulmonary allograft transplantation was finished successfully.The total time of surgical procedure was(65±4)min.The operation successful rate was 85%.By blood gas and pathologic study we successfully found the typical lung ischemia reperfusion injury model.CONCLUSION It is an economical and successful model in highly imitating clinic lung transplantation,which can be used in lung ischemia reperfusion injury.