2012•Chemical Research and ApplicationRequires access

Determination of content of Vitamin C in beverage by HPLC

Jiasheng Xu

Abstract

In this work,HPLC has been used to determine the content of Vitamin C in beverage.ODS C18(4.6mm × 250mm,5μm)is used as reversed phase column.The 0.1%oxalic acid solution is mobile phase.The wavelength of photodiode array detector is 267nm.Its recovery-ratio is 96.4%～100.2%,the correlation coefficient is 0.9963.The relative standard deviation was 0.61%.This HPLC method is simple and convenient,and is easy to control.This analysis method is fast-speed,high sensitivity,and be able to meet requirements of the Vitamin C in beverage industry.

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Available abstract

Key concepts: High-performance liquid chromatography, Chromatography, Oxalic acid, Relative standard deviation, Chemistry, Photodiode, Correlation coefficient, Phase (matter)