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Determination of Vitamin C in Tea Beverages by High Performance Liquid Chromatography

Shan Sheng-ya, Cofco Biochemical

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Abstract

A method was established for determining Vitamin C(Vc) in tea beverages by high performance liquid chromatography(HPLC).The determination was conducted with a ZOBRAX SB-C 18 column used at 28℃.The mobile phase was 0.1% acetic acid solution,a DAD detector was used with a flow rate of 1.0mL/min and a detection wavelength of 250nm.The standard working curve of Vitamin C had a good liner relation in the concentration range from 10 to 300mg/L,and the relative coefficient was 0.9999(n = 7).The recoveries were 99.7% ~ 102%,the RSD were 1.30,0.50,0.92 and 1.21%,respectively,and the detection limit 0.009mg/L.Complying with green chemistry,the method was an accurate and rapid way to determine the content of Vitamin C in tea beverages without toxic and harmful reagents.

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What this paper is about

A method was established for determining Vitamin C(Vc) in tea beverages by high performance liquid chromatography(HPLC).The determination was conducted with a ZOBRAX SB-C 18 column used at 28℃.The mobile phase was 0.1% acetic acid solution,a DAD detector was used with a flow rate of 1.0mL/min and a detection wavelength of 250nm.The standard working curve of Vitamin C had a good liner relation in the concentration range from 10 to 300mg/L,and the relative coefficient was 0.9999(n = 7).The recoveries were 99.7% ~ 102%,the RSD were 1.30,0.50,0.92 and 1.21%,respectively,and the detection limit 0.009mg/L.Complying with green chemistry,the method was an accurate and rapid way to determine the content of Vitamin C in tea beverages without toxic and harmful reagents.

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Available abstract

A method was established for determining Vitamin C(Vc) in tea beverages by high performance liquid chromatography(HPLC).The determination was conducted with a ZOBRAX SB-C 18 column used at 28℃.The mobile phase was 0.1% acetic acid solution,a DAD detector was used with a flow rate of 1.0mL/min and a detection wavelength of 250nm.The standard working curve of Vitamin C had a good liner relation in the concentration range from 10 to 300mg/L,and the relative coefficient was 0.9999(n = 7).The recoveries were 99.7% ~ 102%,the RSD were 1.30,0.50,0.92 and 1.21%,respectively,and the detection limit 0.009mg/L.Complying with green chemistry,the method was an accurate and rapid way to determine the content of Vitamin C in tea beverages without toxic and harmful reagents.

Key concepts: Chromatography, Chemistry, High-performance liquid chromatography, Reagent, Detection limit, Acetic acid, Green tea, Vitamin

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