Determination of Total Flavonoids in Fruit of Pyracantha fortuneana(Maxim.) Li
Gan Xiu
Abstract
Gan Xiu
Abstract
The total flavonoids in the fruit of Pyracantha fortuneana(Maxim.) Li were extracted by Soxhlet extraction method and determined by UV spectrophotometry with rutin as reference standard.The rutin had a good linear relationship in the concentration range of 5—30μg·mL-1,and the correlation coefficient was 0.9997 at 418nm.The method is simple and accurate with good reproducibility,and can be used to determine the content of total flavones in Pyracantha fortuneana(Maxim.)Li.
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The total flavonoids in the fruit of Pyracantha fortuneana(Maxim.) Li were extracted by Soxhlet extraction method and determined by UV spectrophotometry with rutin as reference standard.The rutin had a good linear relationship in the concentration range of 5—30μg·mL-1,and the correlation coefficient was 0.9997 at 418nm.The method is simple and accurate with good reproducibility,and can be used to determine the content of total flavones in Pyracantha fortuneana(Maxim.)Li.
Key concepts: Rutin, Chemistry, Maxim, Flavones, Linear relationship, Chromatography, Reproducibility, Extraction (chemistry)