Systematic Investigation on AlCl_3 Colorimetric Determination of Total Flavonoids from Pyracantha Fortuneana Fruit

Rutin was taken as reference substance and the optimal condition of aluminum chloride colorimetric assay affecting the determination of total flavonoids from Pyracantha fortuneana extract was established. Several parameters, such as the standard substance, light, reaction time and temperature, concentration and the amount of chromogenic agent, p H and buffer dosage that affecting the color reaction of total flavonoids were investigated systematically. The results indicated the appropriate reaction conditions were as the following:2 m L2 %(m/m) Aluminum chloride was added to 1 m L pyracantha extract with specific concentration, after being blended the reaction was carried out at 30 ℃ for 12 min, then absorbance was measured at 285 nm. As a result,Statistical analysis showed the method had good repeatability, stability, reproducibility and high recovery rate.