2012•Jiepou kexue jinzhanRequires access
The effect of recombinant human erythropoietin on the expression of Akt and p-Akt in spinal cord injury rat
Che Min
Open publisher page 0 citations
Abstract
Objective To study the effect of recombinant human erythropoietin(rHuEPO) on the expressions of Akt and p-Akt in spinal cord injury rat. Methods 72 healthy adult Wistar rats were randomly divided into the sham group(n=8), the spinal cord injury group(n=32) and rHuEPO group(n=32). The spinal cord injury was induced with Nystrom's way. The spinal cord injury group and rHuEPO group were further randomly subdivided into four subgroups (6h, 12h, 24h, 3d) according to the postoperative survival time. The expressions of Akt and p-Akt were observed by immunochemistry and Western blot methods. Results The mean optic density(MOD) values of Akt and p-Akt products in spinal cord were decreased in spinal cord injury than in sham group( P0.05), but increased significantly in rHuEPO treated group than in spinal cord injury( P0.05) by immunochemistry and Western blot. Conclusion The impairment effect of rHuEPO in spinal cord injury might be related to the upregulation of the expressions of Akt and p-Akt.