Quantitative Determination of Hyperoside and Quercitrin and Luteolin in Polygonum aviculare by HPLC

ObjectiveThe purpose is to determine the content of hyperoside,quercitrin and luteolin in polygonum aviculare by HPLC.MethodsThe analysis of hyperoside and quercitrin was carried out on Shimadzu C_(18)column(150 mm×4.6 mm,5 μm).The mobile phase was methanol-0.2%phosphate(45∶55).The flow-rate was 1.0 ml·min~(-1)and wave-length was 350 nm.Temperature was room temperature.The analysis of luteolin was carried out on Shimadzu C_(18)column(150 mm×4.6 mm,5 μm).The mobile phase was methanol-0.2% phosphate(50∶50).The flow-rate was 1.0 ml·min~(-1)and wave-length was 254 nm.Temperature was room temperature.ResultsThe method was simple and had a good linear relationship.The linear range of hyperoside was 0.50~2.50 μg and gave a correlation(r) of 0.999 8.The recovery was 95.13%.RSD was 2.91%.The linear range of quercitrin was(0.40)~2.00 μg and gave a correlation(r) of 0.999 6.The recovery was 96.49%.RSD was 2.77%.The linear range of luteolin was(0.40)~2.00 μg and gave a correlation(r) of 0.999 6.The recovery was 95.75%,RSD 2.49%.ConclusionThe method is easy and fast to determine the content of hyperoside,quercitrin and luteolin from polygonum aviculare.