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Quantitative Determination of Hyperoside and Quercitrin in Houttuynia cordata by HPLC

Zheng Yi-min

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Abstract

Objective The purpose is to determine the content of hyperoside and quercitrin in Hout-tuynia cordata. Method The HPLC method was used to determine the content of hyperoside and quercitrin in Houttuynia cordata. The analysis was carried out on Shimadzu C18 column(150 mm×4. 6 mm,5 μm). The mobile phase was MeOH-0. 2%H3PO4 (45:55). Flow-rate was1. 0 mL. min-1. Wave-length was 350 nm. Temperature was room temperature. Result The method was simple and had a good linear relationship. The liner range of hyperoside is 0. 50-2. 50 μg and gave a correlation (r) of 0. 999 8. The recovery was 102. 47%. RSD was 1. 04%. The liner range of quercitrin is 0. 40-2. 00 μg and gave a correlation (r) of 0. 999 6. The recovery was 97. 92%. RSD was 2. 13%. Conclusion The method was easy to determine the content of hyperoside and quercitrin in Houttuynia cordata.

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What this paper is about

Objective The purpose is to determine the content of hyperoside and quercitrin in Hout-tuynia cordata. Method The HPLC method was used to determine the content of hyperoside and quercitrin in Houttuynia cordata. The analysis was carried out on Shimadzu C18 column(150 mm×4. 6 mm,5 μm). The mobile phase was MeOH-0. 2%H3PO4 (45:55). Flow-rate was1. 0 mL. min-1. Wave-length was 350 nm. Temperature was room temperature. Result The method was simple and had a good linear relationship. The liner range of hyperoside is 0. 50-2. 50 μg and gave a correlation (r) of 0. 999 8. The recovery was 102. 47%. RSD was 1. 04%. The liner range of quercitrin is 0. 40-2. 00 μg and gave a correlation (r) of 0. 999 6. The recovery was 97. 92%. RSD was 2. 13%. Conclusion The method was easy to determine the content of hyperoside and quercitrin in Houttuynia cordata.

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Available abstract

Objective The purpose is to determine the content of hyperoside and quercitrin in Hout-tuynia cordata. Method The HPLC method was used to determine the content of hyperoside and quercitrin in Houttuynia cordata. The analysis was carried out on Shimadzu C18 column(150 mm×4. 6 mm,5 μm). The mobile phase was MeOH-0. 2%H3PO4 (45:55). Flow-rate was1. 0 mL. min-1. Wave-length was 350 nm. Temperature was room temperature. Result The method was simple and had a good linear relationship. The liner range of hyperoside is 0. 50-2. 50 μg and gave a correlation (r) of 0. 999 8. The recovery was 102. 47%. RSD was 1. 04%. The liner range of quercitrin is 0. 40-2. 00 μg and gave a correlation (r) of 0. 999 6. The recovery was 97. 92%. RSD was 2. 13%. Conclusion The method was easy to determine the content of hyperoside and quercitrin in Houttuynia cordata.

Key concepts: Quercitrin, Hyperoside, Houttuynia cordata, Chromatography, Chemistry, High-performance liquid chromatography, Quercetin, Antioxidant

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