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2010•Acta Agriculturae Boreali-SinicaRequires access

Cloning and Expression of PGIP Gene from Cucumis melon L.

Huai Feng-tao

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Abstract

A target fragment with a full length of 978 bp was amplified with genomic DNA of Cucumis melon L leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein.The total ORF were comprised by 975 bp of deoxynu-cleotide encoding 325 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Se-quencing analysis showed that it was 100% identical with the sequences of PGIP gene which had been cloned.RT-PCR analysis showed that the PGIP gene was expressed in roots,stems,leaves and fruits of cucumis melon L.As a re-sult,a gene resource was provided for molecular breeding of plants.

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What this paper is about

A target fragment with a full length of 978 bp was amplified with genomic DNA of Cucumis melon L leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein.The total ORF were comprised by 975 bp of deoxynu-cleotide encoding 325 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Se-quencing analysis showed that it was 100% identical with the sequences of PGIP gene which had been cloned.RT-PCR analysis showed that the PGIP gene was expressed in roots,stems,leaves and fruits of cucumis melon L.As a re-sult,a gene resource was provided for molecular breeding of plants.

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Available abstract

A target fragment with a full length of 978 bp was amplified with genomic DNA of Cucumis melon L leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein.The total ORF were comprised by 975 bp of deoxynu-cleotide encoding 325 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Se-quencing analysis showed that it was 100% identical with the sequences of PGIP gene which had been cloned.RT-PCR analysis showed that the PGIP gene was expressed in roots,stems,leaves and fruits of cucumis melon L.As a re-sult,a gene resource was provided for molecular breeding of plants.

Key concepts: Cucumis, Gene, Open reading frame, Biology, Melon, Cloning (programming), Genetics, Sequence analysis

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