Cloning and Tissue Expression Analysis of Two Polygalacturonase Inhibiting Protein Genes from Tobacco

In order to study the biological functions of tobacco PGIP,two full-length polygalacturonase inhibiting protein( PGIP) c DNA and DNA were cloned from Nicotiana tabacum by bioinformation,RT-PCR and SMART RACE technology,which were named Nt PGIP1( Gen Bank Number KF317203) and Nt PGIP2( Gen Bank Number KF317204),respectively. The full length Nt PGIP1 was 1 413 bp which coding a protein for 338 amino acids. The full length Nt PGIP2 was 1 185 bp which coding for 329 amino acids. Sequencing analysis showed that the identical amino acid sequence of the two genes was 54%,while the identical nucleotide sequence was 50%,and no intron sequences were found in the two genes. The amino acid sequences of the two proteins both contained plant PGIP specific conserved leucine repeat sequence LXXLXXLXXLXLXXNXLXGXIPXX. The gene expression patterns were analyzed by Real-time quantitative PCR. The results indicated that the expression of the two genes was detected in all tissues with the highest expression level in stems,followed in roots,and lowest level in leaves.