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Cloning and Bioinformatic Analysis of PGIP Gene from Jiutaiwanli

Yantao Wang

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Abstract

A target fragment with a full length of 1192 bp(GenBank accession:GU068978) was amplified with genomic DNA of Prunus salicina L.cv.Jiutaiwanli leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein,and eontained two exons interrupted by one intron.The total exons were comprised by 990bp of deoxynucleotide encoding 330 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Sequencing analysis showed that it was 95% to 99% identical with the sequences of Prunus PGIP genes including P.salicina,P.armeniaca,P.persica,P.mahaleb,P.mume.Phylogenic tree showed that genetic relationship within the genus was closer and between the genera was farther.As a result,a gene resource was provided for molecular breeding of plants.

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What this paper is about

A target fragment with a full length of 1192 bp(GenBank accession:GU068978) was amplified with genomic DNA of Prunus salicina L.cv.Jiutaiwanli leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein,and eontained two exons interrupted by one intron.The total exons were comprised by 990bp of deoxynucleotide encoding 330 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Sequencing analysis showed that it was 95% to 99% identical with the sequences of Prunus PGIP genes including P.salicina,P.armeniaca,P.persica,P.mahaleb,P.mume.Phylogenic tree showed that genetic relationship within the genus was closer and between the genera was farther.As a result,a gene resource was provided for molecular breeding of plants.

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Available abstract

A target fragment with a full length of 1192 bp(GenBank accession:GU068978) was amplified with genomic DNA of Prunus salicina L.cv.Jiutaiwanli leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein,and eontained two exons interrupted by one intron.The total exons were comprised by 990bp of deoxynucleotide encoding 330 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Sequencing analysis showed that it was 95% to 99% identical with the sequences of Prunus PGIP genes including P.salicina,P.armeniaca,P.persica,P.mahaleb,P.mume.Phylogenic tree showed that genetic relationship within the genus was closer and between the genera was farther.As a result,a gene resource was provided for molecular breeding of plants.

Key concepts: GenBank, Biology, Gene, Genetics, Intron, Exon, Accession number (library science), genomic DNA

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