THE EXPRESSION AND MEANING OF MDM2、p53 PROTEIN IN OVARIAN TUMOR TISSUES AND CELL LINES

Objective: To study the expression and relationship of MDM2 and p53 protein in ovarian tumor tissues? ovarian cancer cell lines. Methods: Cell culture?protein extraction?Western blotting and immunohistochemistry were used. Results: The rate of position expression of MDM2 protein in 17 ovarian cancer cell lines was 11 8%(2/17); the rate of positive expression of p53 was 35 3%(6/17). Two cases of positive expression of MDM2 only appeared in wild type p53, no position in mutation type. In ovarian benign epithelial tumors, borderline epithelial tumors and malignant epithelial tumors, the positive rates of MDN2 and p53 were 20%, 25%, 25%; and 20%, 37 5%, 60% respectively. The positive detection of MDM2 between the three groups of ovarian tumors was not significantly different (P0 05), but the expression of p53 in malignant epithelial tumors was significantly higher than those in the other groups (P0 05). Conclusion: The expression of p53 correlates with the malignant phenotypes of ovarian epithelial tumors. In the pathogenesis and development of malignant ovarian epithelial tumors, the abnormal expression of p53 may be more significant than that of MDM2. MDM2 might play a role in the ovarian cancer without p53 mutation.