Changes and Clinical Significance of Levels of Serum TNF-α,hs-CRP,Adiponectin,HMGB1 in Patients of Type 2 Diabetes Mellitus with Coronary Heart Disease

Objective:To observe the changes of levels of tumor necrosis factor(TNF-α),high sensitivity C-reactive protein(hs-CRP),adiponectin(APN),high mobility group box chromosomal protein 1(HMGB1) in patients of type 2 diabetes mellitus with coronary heart disease,and to explore their clinical significance in monitoring the changes of disease.Methods:A total of 324 cases were enrolled,including patients with diabetes mellitus alone(group A,50 cases),coronary heart disease alone(group B,64 cases),and diabetes mellitus plus coronary heart disease(group C,90 cases),Another 120 normal persons were set as control group.Serum fasting plasma glucose(FPG),and glycation hemoglobin A1c(GHbA1c) were measured.Levels of serum hs-CRP,TNF-α,APN and HMGB1 were detected by enzyme-linked immunosorbent assay(ELISA) andscatter velocity turbidimetry.Results:Levels of FPG,GHbA1c,hs-CRP,TNF-α,and HMGB1 were significantly higher in groups A and C group than in normal control group(all P0.01),while serum adiponectin level in groups A and C was markedly lower than in control group(all P0.01).Levels of hs-CRP,TNF-α,and HMGB1 were significantly higher in group C than that respectively in group A and group B(all P0.01),while serum adiponectin level in group C was markedly lower than in group A and group B(all P0.01).Levels of hs-CRP,TNF-α,and HMGB1 were significantly higher in group B than in group A(all P0.01),serum adiponectin levels in group B were markedly lower than in group A(P0.01).In group C,each of FPG,GHbA1c,and HMGB1 was positively correlated to hs-CRP or TNF-α(all P0.01),while a negative correlation was found between APN and TNF-α or hs-CRP(both P0.01).Pearson correlation analysis showed that Gensini score was positively correlated to HMGB1(P0.01).Conclusion:TNF-α,hs-CRP,adiponectin and HMGB1 participate in the mechanism of atherosclerotic immune pathology in type 2 diabetes mellitus complicated with coronary heart disease,and their measurement are helpful for monitoring disease and guiding treatment.