2010•Food ScienceRequires access
Enzymatic Hydrolysis Processing of Collagen
Shaotong Jiang
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Abstract
Collagen was used as the material to prepare collagen polypeptide through enzymatic hydrolysis by trypsin. The optimal hydrolysis conditions were explored by single factor and response surface experiments on the basis of hydrolysis degree. Results indicated that the optimal hydrolysis conditions were hydrolysis pH of 6.91, hydrolysis temperature of 60 ℃, enzyme dosage of 200 mg per 1 g collagen, material-liquid ratio of 1: 615 (g/mL), and hydrolysis time of 4 h. Under the optimal hydrolysis conditions, the hydrolysis degree of collagen was 29.36%. The solubility and emulsification capability of hydrolyzed collagen were significantly improved.