On Technique Conditions and Parameter of Enzymatic Hydrolysis Porcine Hemoglobin

According to mensuration of enzymatic hydrolysis percentage,the optimization protease was selected and the optimum hydrolysis condition was determined.The hydrolysis condition include the substrate concentration,proportion of enzymes,pH,hydrolysis temperature and hydrolysis times.The result shows that the optimum hydrolysis conditions of enzymatic hydrolysis porcine hemoglobin are as follow follows:the substrate concentration 10%,proportion of enzymes 8‰,pH 7.5,hydrolysis temperature 53℃,hydrolysis times 10h.