THE STUDY ON KILLING PROTOSCOLEX ACTIVITY AND ANTI-INFLAMMATORY OF ALKALOIDS EXTRACTED FROM SOPHORA MOORCROFTIANA SEEDS

Objective To study the efficacy of killing Echinococcus granulosua protoscolex in vitro and anti-inflammatory effect, a-cute toxicity experiment in mice of alkaloids extracted from Sophora moorcroftiana seeds. Methods E. granulosua protoscolex was cultured with alkaloids at 0. 75-6. 00 mg/ml in RPMI-1640 and mortality was counted by methylene blue stain;Kunming mice were injected intraperitoneally with alkaloids to increase progressively from 150 mg/kg, mice had been observed for 14 days and LD50 result performed with NDST statistical software; Using the models of dimethylbenzene-induced mouse ear swelling, the anti-inflammatory effect of alkaloids was evaluated. Results The alkaloids extracted from S. moorcroftiana seeds (0. 75-6. 0 mg/ml) had obviously action of killing E. granulosua protoscolex in vitro. The insecticidal activity was gradually enhanced with a alkaloids variety of time-and-dose. LD50 for mice was (207. 81±20. 82) mg/kg. The alkaloids had no suppressive effect on mouse ear swelling by dimethylbenzene-induced. Conclusion The alkaloids has obviously insecticidal action in vitro and has midding intensive toxicity on mice, and no anti-inflammatory effect on mouse ear swelling by dimethylbenzene-induced.