Construction and identification of the eukaryotic expression vector of IL-37(pIRES2-EGFP/IL-37)

Objective To construct and identify the eukaryotic expression vector of IL-37(p IRES2-EGFP/IL-37). Methods RNA was extracted from THP-1 cells and IL-37 gene was amplified by RT-PCR. The target gene was cloned into the eukaryotic expression vector p IRES2-EGFP. The recombinant plasmid was transfected into Dh5a strain of Escheria coli. The p IEIL37 was identified by PCR, double enzyme digestion and DNA sequencing. Results Agarose electrophoresis revealed a 710 bp band. Double enzyme digestion showed a 675 bp band. DNA sequencing indicated the insertion of IL-37 gene into eukaryotic expression vector p IRES2-EGFP. Conclusion The eukaryotic expression vector of IL-37(p IRES2-EGFP/IL-37) is successfully constructed.