Inhibitory effect of JA1 on H_(22) cells in vitro and in vivo

To investigate the inhibitory effect of the extractive of Ammopiptanthus mongolicus(JA1)on hepatocarcinoma cells and its possible mechanisms,the hepatocellular carcinoma cell line of mice(H22)was used as target cell.The inhibitory effects of JA1 on H22 cells were assayed by MTT in vitro and the antitumor effect of JA1 was also observed in H22-bearing mice by flow cytometry assays,light and transmission electronic microscopy.The results revealed that JA1 had significant anti-proliferative effects on H22 cells with the dependence of time and dosage.In addition,JA1 not only significantly inhibited the proliferation of H22 cells in vitro but also inhibited the growth of H22 hepatocarcinoma in vivo.Flow cytometric analysis indicated that JA1 could induce apoptosis on H22 cells in H22-bearing mice and the G1 phase cell percentage increased and the S phase cell percentage decreased.Morphological change of H22 cells undergoing apoptosis were also detected by light and electron microscopy.These results suggest that JA1 has better inhibitory effects on H22in vitro and vivo and the mechanism maybe related to its apoptosis inducing activity.