Apoptosis of Hep cells induced by JA1 in vitro and in vivo

To investigate the inhibitory effect of the extractive of Ammopiptanthus mongolicus(JA1)on hepatocarcinoma cells and its possible mechanisms,the hepatocellular carcinoma cell line of mice(Hep)was used as target cell.The inhibitory effects of JA1 on Hep cells were assayed by MTT,apoptosis were measured by flow cytometry in vitro and the antitumor effect of JA1 was also observed in Hep-bearing mice by flow cytometry assay,light microscopy and transmission electronic microscopy.JA1 had significant anti-proliferative effects on Hep cells with dependence of time and dosage.In addition,JA1 not only could inhi-bit significantly the proliferation of Hep cells in vitro but also inhibit the growth of Hep hepatocarcinoma in vivo.Flow cytometric analysis indicated that JA1 could induce apoptosis on Hep cells in Hep-bearing mice and the G1 phase cell percentage was incrassation and the S phase cell percentage decrease.Morphological change of Hep cells undergoing apoptosis were also detected by light and electron microscopies.These result showed that JA1 had good inhibitory effects on Hep in vitro and in vivo and the mechanism was related to its apoptosis inducing activity.