STUDY ON TOXICITY AND ACTIVITY OF SOD AND MDA OF PROPOLIS.

Objective:To study the toxicity of propolis and its effect on free radical in NIH mice.Methods:Acute toxicity test,Micronucleus test of bone marrow cell,the test of sperm shape abnormality in mice and 30 days feeding test were carried out,the content of SOD and MDA was determined with related reagent kit.Result:Animal experiment demonstrated that the LD 50 of propolis is 20000mg/Kg (oral to NIH mice).Micronucleus test,the test of sperm shape abnormality and thirty days feeding test showed that propolis has not significant difference on toxicity compared with the control group ( P 0 05).The activity of SOD in all experimental groups was higher than that of control group( P 0 01) whereas the higher concentrations resulted in a concentration related increase.The MDA content of all experiment groups was lower than that of control group,but no significantly different compared with the control( P 0 05).Conclusions:The results determined that propolis is a kind of safe, non toxic healthy food.The further study is necessary.