Separation and purification of flavonoids from Artemisia argyi with macroporous resin
Sun Zhida
Abstract
Sun Zhida
Abstract
A method for separation and purification of flavonoids from Artemisia argyi with macroporous resin was studied,with the content and recovery rate of flavonoids as indexes.The results are as follows:AB-8 type macroporous resin is the best for separating and purificating Artemisia argyi flavonoids;the optimun conditions is:the loaded amount of Artemisia argyi extract is 4 mL,wash with water(pH 5.0) ,the concentration of desorption agent is 50%(pH 5.0) ethanol.The AB-8 type resin can be used 3 times repeatedly.The content of flavonoids in the 50% ethanol eluant is 69.37% with the recovery rate of 64.98% after purification with AB-8 type resin.
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A method for separation and purification of flavonoids from Artemisia argyi with macroporous resin was studied,with the content and recovery rate of flavonoids as indexes.The results are as follows:AB-8 type macroporous resin is the best for separating and purificating Artemisia argyi flavonoids;the optimun conditions is:the loaded amount of Artemisia argyi extract is 4 mL,wash with water(pH 5.0) ,the concentration of desorption agent is 50%(pH 5.0) ethanol.The AB-8 type resin can be used 3 times repeatedly.The content of flavonoids in the 50% ethanol eluant is 69.37% with the recovery rate of 64.98% after purification with AB-8 type resin.
Key concepts: Chemistry, Chromatography, Ethanol, Artemisia, Elution, Desorption, Recovery rate, Adsorption