A Study on the Purification of Flavonoids from Artemisia Annua L. by Macroporous Resins and Their Antioxidant Activity

The purification of flavonoids from Artemisia Annua L.by macroporous adsorption resins was studied and the antioxidant activity of the flavonoids was investigated.The adsorption and desorption effects of AB-8,DM-101,DA-201,D-101,SD-401 on flavonoids from Artemisia Annua L.were compared.The results show that the D-101 macroporous resin has the best adsorption and desorption effect.Through the investigation into the dynamic parameters of adsorption and desorption of D-101 macroporous resin,the optimal purification conditions were ascertained.For adsorption,the optimal conditions were:the sample mass concentration of flavonoids 1.55 g/L,flow velocity 2 mL/min,sample volume 100 mL,then a wash-out by distilled water till the solution became colorless.For desorption,the optimal conditions were:eluant concentration 70% ethanol,eluant volume 250 mL,flow velocity of eluant 1 mL/min.Under the optimal conditions,the content of flavonoids is raised from 20.10% to 80.32%,the ratio of desorption reaches 91.08%,and the yield of total flavones is 68.25%.The flavonoids from Artemisia Annua L.exhibit obvious antioxidant effect on oil and fat.The antioxidant activity of flavonoids from Artemisia Annua L.on vegetable oil is superior to that of both ascorbic acid and citric acid,yet it is somewhat superior to that of citric acid but inferior to that of ascorbic acid when it comes to animal fat.