Purification of Total Flavonoids from Artemisia Argyi by Macroreticular Resin

Aim To study the technology for purification of total flavonoids from artemisia argyi with different types of macroporous resin.Methods The static capacity adsorption and static elution ratio of 5 types of macroporous resin were studied respectively and compared to find the optimum one.The content of total flavonoids was researched and compared to obtain optimum technological parameters.Results the AB-8 type of macroporous resin showed optimum adsorption and elution parameters.The concentration of artemisia argyi sample extract was 4.42mg·mL-1 and its pH was 4.0.After being eluted with 3mL/min of 70% ethol,the yield of total flavonoids was 82% and the product content was 64%.Conclusion The AB-8 showed better comprehensive adsorption and could be used to separate and purity the extraction of total flavonoids from artemisia argyi.