Purification of Total Alkaloid from Rhizoma Coptidis by Weakly Acidic Ion Exchange Resin

Objective:To investigate the purification technology of total alkaloids from Rhizoma Coptidis by macroporous resin,and to provide feasible program for large-scale manufacture.Method:Evaluated the effect of purification of total alkaloids from Rhizoma Coptidis by D101,AB-8 and D151 resins respectively with the indicator of the content of berberine.Result:It has been found that D151 resin's adsorption capacity of total alkaloids was the best in those three resins.The optimum process was as follows:the concentration of the sample solution was 0.15 g·L-1 with 9 times the amount of resin volume,washed 2 times the amount of resin volume by distilled water and 5 times the amount of resin volume by 0.12 mol·L-1 of HCl solution.Under those conditions,more than 60% extract was reduced with the proportion of total alkaloid in excess of 80%,and 70% of sample recover.Conclusion:D151 resin is suitable for industrial production for excellent drug loading and purification.