Establishment of Rat Model of Type 2 Diabetes

Objective To establish type 2 diabetic model in rats with streptozotocin and high-carbonhydrate-fat diet.Methods Thirty male rats were randomly divided into experimental group and control group,and twenty rats in experimental group were fed with high-carbonhydrate-fat diet and ten rats in control group were raised by basic diet.The rats in experimental group were divided into high-carbonhydrate-fat group and diabetic group.After the rats in high-carbonhydrate-fat group were raised for three weeks,the blood was collected to detect the blood glucose and serum insulin levels.The rats in diabetic group received one-off intraperitoneal injection of streptozotocin at a dose of 25 mg/(kg·BW),and underwent glucose toterance test to authenticate abnormality after three days,and then were fed continually with high-carbonhydrate-fat feeds.Hemospasia was used again to detect blood glucose and serum insulin levels in diabetic rats in the second and forth week.The rats in control group were intraperitoneally injected with corresponding dose of citrate sodium buffer solution and the method of collecting blood was the same as the experimental group.The changes of glucose toterance,blood glucose and serum insulin levels were compared between the groups.Results Compared with control group,the serum insulin levels in the high-carbonhydrate-fat group were increased significantly(P0.01),but the blood glucose levels weren't changed(P0.05).The blood glucose and serum insulin levels in diabetic group were both increased significantly as compared with control group(all P0.01).Conclusion The obvious hyperinsulinism were produced in the rats which were fed with high-carbonhydrate-fat diet,and the glucose tolerance abnormity was caused by high-carbonhydrate-fat assisted with low-dose intraperitoneal injection of streptozotocin.In this way,the type 2 diabetic animal model in rats can be induced successfully.