Optimization of extraction of protein from base of Flammulina velutipes stipe by ultrasonic

Flammulina velutipes scraps are rich in proteins and bioactive substances. In order to fully utilize the leftovers of Flammulina velutipes,protein in base of Flammulina velutipes stipe was extracted. Response surface methodology was used to optimize the ultrasonic-assisted extraction processing of protein from base of Flammulina velutipes stipe for achieving maximum extraction rate of proteins. The optimum extraction parameters obtained from response surface analysis were as follows: ultrasonic power 124. 33 W,extraction temperature 29. 40℃,ultrasonic time 19. 76 min. By using the optimized extraction parameters,the predicated extraction rate of proteins was 73. 02%,meanwhile the rate of proteins was 73. 22% in the validated experiment with the relative deviation value of 0. 27%. There was no significant difference between predictive value and actual value.