The diagnose value of urinary MCP-1 and plasma interleukin-8 in purpura nephritis

Objective:To explore the diagnose value of the urine monocyte chemoattractant protein-1(MCP-1) and plasma interleukin-8(IL-8) in purpura nephritis.Methods:There were 57 cases who suffered from Henoch-Schonlein purpura included in the experimental group,including 45 cases complicated with purpura nephritis.According to the level of urinary albumin,they were divided into 4 groups:group 1(normal urinary albumin),group 2(micro urinary albumin),group 3(a large number of urinary albumin),group 4(simple purpuric group with normal urine).In the same period,19 cases of healthy children were selected as control group.The urinary MCP-1,and plasma IL-8 were examined in all cases by ELISA.The differences of urinary MCP-1,plasma IL-8 levels were compared among all the group,and the areas under the ROC curves were caculated.Results:The comparative differences of urine MCP-1 level from experimental group 1 to 4 had statistical significance(F = 88.52,P 0.01).With the increase of urinary albumin,the urine MCP-1 level increased altogether.The differences from comparisons between one group and another all had statistical significance(P 0.01).The comparative differences of plasma IL-8 levels from experimental group 1 to 4 and the control group had statistical significance(F = 289.55,P 0.01).Comparisons between one group and another indicate:The comparative differences of plasma IL-8 level in urine albumin groups(group 1 to 3) of different levels had statistical significance(P 0.01),whereas the comparative difference of normal urinary albumin group(group 1) and simple purpura group(group 4) did not have statistical significance(P 0.05).The plasma IL-8 level of experimental groups(group 1 to 4) was obviously higher than that of control groups in average(P 0.01).The areas under ROC curvs of urine MCP-1 and plasma IL-8 were 0.90 and 0.83.Their diagnostic boundary values were 111.50 ng / L and 349.54 ng / L and their sensitivities were 82.22% and 68.89%.Conclusion:The sensitivity of urine MCP-1 to the early diagnosis of Henoch-Schonlein purpura nephritis(HSPN) was superior to that of plasma IL-8.And these two are both helpful for the judgment of the severity of HSPN damage.